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Scientist - Biology Job Responsibilities - Design, develop, and optimize in vitro and biochemical (cell-based and purified protein) assays to characterize drug–target interactions for target validation, compound screening, and mechanism-of-action studies.
- Apply equilibrium/kinetic binding models to derive parameters (Kd, Ki, kon, koff, residence time, stoichiometry) using radiometric and fluorescence platforms.
- Plan experiments to distinguish specific vs. non-specific binding; control receptor expression and probe depletion/rebinding.
- Analyze cellular target engagement data (structure-function, conformational dynamics, MoA).
- Use statistical/computational methods to ensure assay robustness and reproducibility (e.g., Z’ factor, CV, signal window).
- Translate scientific questions into robust assays; lead projects supporting the biology screening/evaluation plan and collaborate with chemists, DMPK, imaging, and in vivo teams. ...